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fixup! * When running differential expression or feature counting, RN…
…Alysis session reports will automatically include a logfile with R session info. * Added optional parameters to all differential expression functions, allowing users to return a path to a logfile with R session info.
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..._pipeline_tests/paired/truth/01_trim_adapters_paired_end/cutadapt_log_reads_1_reads_2.log
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This is cutadapt 4.9 with Python 3.12.3 | ||
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Command line parameters: -a ATGGGTATATGGGT -A AGTTTACCGTTGT --quality-cutoff 20 --minimum-length 10 --trim-n --cores 0 --overlap 3 --error-rate 0.1 --pair-filter=any --output tests/test_files/fastq_pipeline_tests/paired/outdir/01_trim_adapters_paired_end/reads_1_trimmed.fastq --paired-output tests/test_files/fastq_pipeline_tests/paired/outdir/01_trim_adapters_paired_end/reads_2_trimmed.fastq tests/test_files/fastq_pipeline_tests/in/reads_1.fastq tests/test_files/fastq_pipeline_tests/in/reads_2.fastq | ||
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Processing paired-end reads on 12 cores ... | ||
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Finished in 2.095 s (209.489 �s/read; 0.29 M reads/minute). | ||
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=== Summary === | ||
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Total read pairs processed: 10,000 | ||
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Read 1 with adapter: 163 (1.6%) | ||
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Read 2 with adapter: 185 (1.8%) | ||
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== Read fate breakdown == | ||
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Pairs that were too short: 3 (0.0%) | ||
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Pairs written (passing filters): 9,997 (100.0%) | ||
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Total basepairs processed: 1,000,112 bp | ||
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Read 1: 500,042 bp | ||
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Read 2: 500,070 bp | ||
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Quality-trimmed: 0 bp (0.0%) | ||
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Read 1: 0 bp | ||
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Read 2: 0 bp | ||
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Total written (filtered): 998,560 bp (99.8%) | ||
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Read 1: 499,313 bp | ||
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Read 2: 499,247 bp | ||
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=== First read: Adapter 1 === | ||
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Sequence: ATGGGTATATGGGT; Type: regular 3'; Length: 14; Trimmed: 163 times | ||
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Minimum overlap: 3 | ||
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No. of allowed errors: | ||
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1-9 bp: 0; 10-14 bp: 1 | ||
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Bases preceding removed adapters: | ||
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A: 28.8% | ||
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C: 37.4% | ||
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G: 17.8% | ||
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T: 14.1% | ||
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none/other: 1.8% | ||
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Overview of removed sequences | ||
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length count expect max.err error counts | ||
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3 116 156.2 0 116 | ||
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4 32 39.1 0 32 | ||
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5 11 9.8 0 11 | ||
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6 1 2.4 0 1 | ||
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64 3 0.0 1 3 | ||
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=== Second read: Adapter 2 === | ||
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Sequence: AGTTTACCGTTGT; Type: regular 3'; Length: 13; Trimmed: 185 times | ||
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Minimum overlap: 3 | ||
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No. of allowed errors: | ||
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1-9 bp: 0; 10-13 bp: 1 | ||
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Bases preceding removed adapters: | ||
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A: 27.0% | ||
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C: 28.1% | ||
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G: 31.4% | ||
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T: 13.5% | ||
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none/other: 0.0% | ||
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Overview of removed sequences | ||
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length count expect max.err error counts | ||
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3 124 156.2 0 124 | ||
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4 40 39.1 0 40 | ||
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5 18 9.8 0 18 | ||
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6 1 2.4 0 1 | ||
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7 1 0.6 0 1 | ||
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10 1 0.0 1 0 1 | ||
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tests/test_files/fastq_pipeline_tests/paired/truth/03_featurecounts_paired_end/logfile.log
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========== _____ _ _ ____ _____ ______ _____ | ||
===== / ____| | | | _ \| __ \| ____| /\ | __ \ | ||
===== | (___ | | | | |_) | |__) | |__ / \ | | | | | ||
==== \___ \| | | | _ <| _ /| __| / /\ \ | | | | | ||
==== ____) | |__| | |_) | | \ \| |____ / ____ \| |__| | | ||
========== |_____/ \____/|____/|_| \_\______/_/ \_\_____/ | ||
Rsubread 2.14.2 | ||
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//========================== featureCounts setting ===========================\\ | ||
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|| Input files : 1 SAM file || | ||
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|| reads_.sam || | ||
|| || | ||
|| Paired-end : yes || | ||
|| Count read pairs : yes || | ||
|| Annotation : transcripts.gtf (GTF) || | ||
|| Dir for temp files : . || | ||
|| Threads : 1 || | ||
|| Level : meta-feature level || | ||
|| Multimapping reads : not counted || | ||
|| Multiple alignments : primary alignment only || | ||
|| Multi-overlapping reads : not counted || | ||
|| Min overlapping bases : 1 || | ||
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\\============================================================================// | ||
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//================================= Running ==================================\\ | ||
|| || | ||
|| Load annotation file transcripts.gtf ... || | ||
|| Features : 79 || | ||
|| Meta-features : 11 || | ||
|| Chromosomes/contigs : 2 || | ||
|| || | ||
|| Process SAM file reads_.sam... || | ||
|| Paired-end reads are included. || | ||
|| Total alignments : 9997 || | ||
|| Successfully assigned alignments : 0 (0.0%) || | ||
|| Running time : 0.00 minutes || | ||
|| || | ||
|| Write the final count table. || | ||
|| Write the read assignment summary. || | ||
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\\============================================================================// | ||
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R version 4.3.1 (2023-06-16 ucrt) | ||
Platform: x86_64-w64-mingw32/x64 (64-bit) | ||
Running under: Windows 11 x64 (build 22631) | ||
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Matrix products: default | ||
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locale: | ||
[1] LC_COLLATE=English_United States.utf8 | ||
[2] LC_CTYPE=English_United States.utf8 | ||
[3] LC_MONETARY=English_United States.utf8 | ||
[4] LC_NUMERIC=C | ||
[5] LC_TIME=English_United States.utf8 | ||
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time zone: Asia/Jerusalem | ||
tzcode source: internal | ||
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attached base packages: | ||
[1] stats graphics grDevices utils datasets methods base | ||
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other attached packages: | ||
[1] Rsubread_2.14.2 | ||
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loaded via a namespace (and not attached): | ||
[1] compiler_4.3.1 Matrix_1.6-1.1 grid_4.3.1 lattice_0.21-9 |
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...es/fastq_pipeline_tests/single/truth/01_trim_adapters_single_end/cutadapt_log_reads_1.log
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This is cutadapt 4.9 with Python 3.12.3 | ||
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Command line parameters: --adapter ATGGGTATATGGGT --quality-cutoff 20 --minimum-length 10 --trim-n --cores 0 --overlap 3 --error-rate 0.1 --output tests/test_files/fastq_pipeline_tests/single/outdir/01_trim_adapters_single_end/reads_1_trimmed.fastq tests/test_files/fastq_pipeline_tests/in/reads_1.fastq | ||
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Processing single-end reads on 12 cores ... | ||
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Finished in 2.048 s (204.823 �s/read; 0.29 M reads/minute). | ||
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=== Summary === | ||
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Total reads processed: 10,000 | ||
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Reads with adapters: 163 (1.6%) | ||
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== Read fate breakdown == | ||
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Reads that were too short: 3 (0.0%) | ||
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Reads written (passing filters): 9,997 (100.0%) | ||
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Total basepairs processed: 500,042 bp | ||
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Quality-trimmed: 0 bp (0.0%) | ||
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Total written (filtered): 499,313 bp (99.9%) | ||
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=== Adapter 1 === | ||
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Sequence: ATGGGTATATGGGT; Type: regular 3'; Length: 14; Trimmed: 163 times | ||
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Minimum overlap: 3 | ||
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No. of allowed errors: | ||
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1-9 bp: 0; 10-14 bp: 1 | ||
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Bases preceding removed adapters: | ||
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A: 28.8% | ||
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C: 37.4% | ||
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G: 17.8% | ||
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T: 14.1% | ||
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none/other: 1.8% | ||
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Overview of removed sequences | ||
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length count expect max.err error counts | ||
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3 116 156.2 0 116 | ||
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4 32 39.1 0 32 | ||
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5 11 9.8 0 11 | ||
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6 1 2.4 0 1 | ||
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64 3 0.0 1 3 | ||
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80 changes: 80 additions & 0 deletions
80
...es/fastq_pipeline_tests/single/truth/01_trim_adapters_single_end/cutadapt_log_reads_2.log
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Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,80 @@ | ||
This is cutadapt 4.9 with Python 3.12.3 | ||
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Command line parameters: --adapter ATGGGTATATGGGT --quality-cutoff 20 --minimum-length 10 --trim-n --cores 0 --overlap 3 --error-rate 0.1 --output tests/test_files/fastq_pipeline_tests/single/outdir/01_trim_adapters_single_end/reads_2_trimmed.fastq tests/test_files/fastq_pipeline_tests/in/reads_2.fastq | ||
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Processing single-end reads on 12 cores ... | ||
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Finished in 2.189 s (218.890 �s/read; 0.27 M reads/minute). | ||
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=== Summary === | ||
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Total reads processed: 10,000 | ||
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Reads with adapters: 164 (1.6%) | ||
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== Read fate breakdown == | ||
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Reads that were too short: 5 (0.1%) | ||
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Reads written (passing filters): 9,995 (100.0%) | ||
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Total basepairs processed: 500,070 bp | ||
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Quality-trimmed: 0 bp (0.0%) | ||
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Total written (filtered): 499,234 bp (99.8%) | ||
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=== Adapter 1 === | ||
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Sequence: ATGGGTATATGGGT; Type: regular 3'; Length: 14; Trimmed: 164 times | ||
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Minimum overlap: 3 | ||
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No. of allowed errors: | ||
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1-9 bp: 0; 10-14 bp: 1 | ||
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Bases preceding removed adapters: | ||
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A: 27.4% | ||
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C: 29.9% | ||
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G: 21.3% | ||
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T: 18.3% | ||
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none/other: 3.0% | ||
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Overview of removed sequences | ||
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length count expect max.err error counts | ||
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3 127 156.2 0 127 | ||
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4 26 39.1 0 26 | ||
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5 5 9.8 0 5 | ||
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6 1 2.4 0 1 | ||
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64 5 0.0 1 5 | ||
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