diff --git a/tests/drivers/mspi/api/boards/nrf54l15dk_nrf54l15_cpuapp.overlay b/tests/drivers/mspi/api/boards/nrf54l15dk_nrf54l15_cpuapp.overlay
new file mode 100644
index 00000000000..84115ac7de6
--- /dev/null
+++ b/tests/drivers/mspi/api/boards/nrf54l15dk_nrf54l15_cpuapp.overlay
@@ -0,0 +1,21 @@
+/*
+ * Copyright (c) 2024 Ambiq Micro Inc. <www.ambiq.com>
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+/ {
+	aliases {
+		mspi0 = &emspi;
+	};
+};
+
+&emspi {
+	status = "okay";
+
+	mspi_device: mspi_device@0 {
+		status = "okay";
+		compatible = "zephyr,mspi-emul-device";
+		reg = <0x0>;
+		mspi-max-frequency = <48000000>;
+	};
+};
diff --git a/tests/drivers/mspi/api/testcase.yaml b/tests/drivers/mspi/api/testcase.yaml
index 3675cc485f3..924880c5f57 100644
--- a/tests/drivers/mspi/api/testcase.yaml
+++ b/tests/drivers/mspi/api/testcase.yaml
@@ -15,3 +15,18 @@ tests:
       - apollo3p_evb
     integration_platforms:
       - native_sim
+
+  drivers.mspi.api.emspi:
+    tags:
+      - drivers
+      - mspi
+      - api
+    harness: ztest
+    platform_allow:
+      - nrf54l15dk_nrf54l15_cpuapp
+    integration_platforms:
+      - nrf54l15dk_nrf54l15_cpuapp
+    extra_args:
+      - SB_CONFIG_PARTITION_MANAGER=n
+      - SB_CONFIG_SDP=y
+      - SB_CONFIG_SDP_MSPI=y