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running problems with graph-diff #165

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MingjingKang opened this issue Nov 21, 2020 · 0 comments
Open

running problems with graph-diff #165

MingjingKang opened this issue Nov 21, 2020 · 0 comments

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@MingjingKang
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MingjingKang commented Nov 21, 2020

Hello Jared,

After ran “preprocess”, “correct”, "filter", "overlap" and “assemble” with default settings, I got two contigs files for reference genome ref_contigs.fa and query genome query_contigs.fa. When I tried to run "graph-diff" with the following command to call the variants,

sga graph-diff -p D0D3 -t 16 -k 61 -x 10 --variant query_contigs.fa --ref ref_contigs.fa

it kept reporting wrong message:

_Error: unrecognized algorithm string:

Usage: sga graph-diff [OPTION] --base BASE.fa --variant VARIANT.fa --ref REFERENCE.fa
Find and report strings only present in the graph of VARIANT when compared to BASE_

I tried all possible commands, but they didn't work. I installed SGA by conda.
Could you tell me what's wrong with the command?

Thanks a lot,
Ming

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